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Kala Kumar, B.
- Protective Effect of Rutin in Comparison to Silymarin Against Induced Hepatotoxicity in Rats
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Materials and Methods: Male Wistar albino rats (n=24) of 3 months age were equally divided into four groups. Group 1 served as normal control. Hepatotoxicity was induced in the remaining three groups with administration of 500 mg/kg po APAP from day 1-3. Groups 2, 3, and 4 were subsequently administered orally with distilled water, 25 mg/kg of SLM, and 20 mg/kg of RTN, respectively, for 11 days. The mean body weights and biomarkers of hepatotoxicity were estimated on day 0, 4 (confirmation of toxicity), and 15 (at the end of treatment). Hematological parameters were evaluated on day 4 and 15. Antioxidant profile and adenosine triphosphatases (ATPases) were assessed at the end of the experiment. Liver tissues were subjected to histopathology and transmission electron microscopy after the sacrifice on day 15.
Results: Antioxidant profile, ATPases, and hematological and sero-biochemical parameters were significantly altered, and histopathological changes were noticed in the liver of toxic control group. These changes were reversed in groups 3 and 4 that were administered with SLM and RTN, respectively.
Conclusion: The results of the present investigation enunciated that SLM has potent hepatoprotective activity though the RTN was found superior in restoring the pathological alterations in paracetamol-induced hepatotoxicity in Wistar albino rats.
Authors
M. Kasi Reddy
1,
A. Gopala Reddy
1,
B. Kala Kumar
1,
D. Madhuri
2,
G. Boobalan
1,
M. Anudeep Reddy
1
Affiliations
1 Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, IN
2 Department of Veterinary Pathology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, IN
1 Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, IN
2 Department of Veterinary Pathology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, IN
Source
Veterinary World, Vol 10, No 1 (2017), Pagination: 74-80Abstract
Aim: The aim of this study is to evaluate the hepatoprotective effect of rutin (RTN) in comparison to silymarin (SLM) against acetaminophen (APAP)-induced hepatotoxicity in rats.Materials and Methods: Male Wistar albino rats (n=24) of 3 months age were equally divided into four groups. Group 1 served as normal control. Hepatotoxicity was induced in the remaining three groups with administration of 500 mg/kg po APAP from day 1-3. Groups 2, 3, and 4 were subsequently administered orally with distilled water, 25 mg/kg of SLM, and 20 mg/kg of RTN, respectively, for 11 days. The mean body weights and biomarkers of hepatotoxicity were estimated on day 0, 4 (confirmation of toxicity), and 15 (at the end of treatment). Hematological parameters were evaluated on day 4 and 15. Antioxidant profile and adenosine triphosphatases (ATPases) were assessed at the end of the experiment. Liver tissues were subjected to histopathology and transmission electron microscopy after the sacrifice on day 15.
Results: Antioxidant profile, ATPases, and hematological and sero-biochemical parameters were significantly altered, and histopathological changes were noticed in the liver of toxic control group. These changes were reversed in groups 3 and 4 that were administered with SLM and RTN, respectively.
Conclusion: The results of the present investigation enunciated that SLM has potent hepatoprotective activity though the RTN was found superior in restoring the pathological alterations in paracetamol-induced hepatotoxicity in Wistar albino rats.
Keywords
Acetaminophen, Hepatotoxicity, Rutin, Silymarin.References
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- Modulation of Oxidative Stress Mediators in Adjuvant Induced Arthritis by Herbal Adaptogen Boswellia serrata
Abstract Views :199 |
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Authors
Affiliations
1 JRF, ICAR-NDRI, Southern Regional Station, Adugodi, Bengaluru – 560030, Karnataka, IN
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telangana, IN
3 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Korutla, Kanchikacherla - 505326, Telangana, IN
1 JRF, ICAR-NDRI, Southern Regional Station, Adugodi, Bengaluru – 560030, Karnataka, IN
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telangana, IN
3 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Korutla, Kanchikacherla - 505326, Telangana, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 25, No 4 (2018), Pagination: 193-197Abstract
A study was carried out to evaluate Boswellia serrata on adjuvant induced oxidative stress in rats. Intradermal administration of Freund’s adjuvant to induce rheumatoid arthritis consequently produced oxidative damage as indicated by an increase in thiobarbituric acid reducing substances, myeloperoxidase, protein carbonyls and a reduction in superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione in tissue homogenates. Oral administration of methanolic extracts ofBoswellia serrata at 500 mg/kg body weight re-established the antioxidant profile. Co-treatment with an anti-inflammatory agent, meloxicam showed an enhanced healing effect. Hence, a significant restorative effect was witnessed with the herbal adaptogen Boswellia serrata.Keywords
Adjuvant Arthritis, Antioxidants, Boswellia serrata, Oxidative Stress.References
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